| Title | [Cloning and analysis of promoter-active fragments from Corynebacterium glutamicum 10147] | | Author(s) | Liu G, Zhao Z, Zhang Y, Wang Y, Ding J | | Institution | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. guimingl@126.com | | Source | Wei Sheng Wu Xue Bao 2009 Jul 4; 49(7):972-7. | | Abstract | OBJECTIVE: To clone promoter-active fragments from Corynebacterium glutamicum for further construction of expression vectors.
METHODS: Random Sau3A I digested fragments of C. glutamicum 10147 chromosome were shot-gun cloned into the promoter-probe vector pAKC6 and promoter activity of the inserted fragments was selected by chloramphenicol resistance of transformed C. glutamicum cells.
RESULTS: Thirty promoter-carrying fragments were isolated. Three C. glutamicum clones harboring pAKC6 with promoter fragments displayed chloramphenicol acetyltransferase (CAT) activity of more than 24 U/mg. The fragment F57 led to the highest CAT activity of 32.50 U/mg, even more than that produced by the promoter Ptrc, 26.33 U/mg.
CONCLUSION: The strength of promoter on fragments F21, F54 and F57 is as strong as promoter Ptrc in C. glutamicum. These fragments can be used to construct expression vector. | | Language | chi | | Pub Type(s) | English Abstract
Journal Article
| | PubMed ID | 19873765 |
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